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Protein Crystallisation in Action

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Protein Crystallisation in Action

by Dima Chirgadze

July 3, 2001



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Abstract:

A critical phase in an X-ray structural determination process is the crystallisation of the purified protein. Not only is this step time-consuming, it also requires a considerable amount of protein sample. The main objective is to obtain large, preferably single well-ordered crystals capable of diffracting X-rays to the highest possible resolution. In this paper I will describe the theory, physical methods and strategy involved in the crystallisation of biological macromolecules, and also the work that has been done to crystallise two distinct proteins - HR1b domain of PRK1 (Protein Kinase C-Related Kinase 1) and NK1 fragment of HGF/SF (Hepatocyte Growth Factor/Scatter Factor).



 




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2001-07-04