Molecular Replacement in Action
Molecular Replacement in Action.
The NK1 fragment crystal structure solution by Molecular Replacement
by Dima Chirgadze
July 3, 2001
When identical or similar structures exist in different crystallographic
environments (like space group, unit cell parameters), similarities
between their diffraction patterns, which are directly associated
with the Fourier transforms of these structures, would be expected.
The technique of Molecular Replacement in protein X-ray crystallography
utilises this similarity in order to derive the initial phase information.
This paper introduces the theory underlying the Molecular Replacement
method as applied to the solution of protein crystal structures. It
also describes the application of this method to the NK1 fragment
crystal structure solution using the NMR model of HGF/SF (Hepatocyte
Factor) N-domain and the crystal structure of human plasminogen
kringle 1 domain as molecular replacement search probes.
Copyright © 1999-2001 by Dima Chirgadze,