Instruments


X-ray Diffraction System:

ICARUS: X8 PROTEUM (by Bruker AXS)  X-ray diffraction system for macromolecular crystallography, comprising of:

  • MISCROSTAR microfocus rotating anode X-ray generator
  • HELIOS MX  high-brightness multilayer X-ray optics
  • PLATINUM135 CCD detector
  • Kappa 4-axis goniometerICARUS-web2
  • COBRA (by Oxford Cryosystems Ltd) – non-liquid nitrogen sample cooler
  • BruNo2 sample changing robot
  • PROTEUM 2 software suite for X-ray diffraction data collection and processing
  • PC running Linux OpenSuse for data collection control and processing

Crystallisation Robotics:

TECAN: Freedom EVO 100 (by Tecan Group Ltd) Liquid Handling Platform – creation of customised crystallisation screens and dispensation of commercial crystallisation kits into a variety of crystallisation plates.

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ORYX: Oryx6 (by Douglas Instruments Ltd) Automatic Protein Crystallisation system for protein crystallisation. Can be used for sitting drop crystallisation, “MMS” Microseed Matrix Screening, 2D optimisation, additive experiments, microbatch-under-oil and multi-variate optimisation.

 

PHOENIX: CRYSTAL PHOENIX (by Art Robbins Instruments, Inc.) Protein Crystallisation System – 96 channel (+1 sample) high-speed liquid dispensing system for automated setup of macromolecular crystallisation trials.

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Crystallisation Imaging System:

ROCK IMAGER 1000 (by Formulatrix, Inc) is an automated imaging system for protein crystallisation. Incubates and captures high quality images of up to 1000 microplates on a user-defined schedule. The system also includes the UV modification allowing users to easily discover which crystals are salt and which are protein.

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Other Crystallisation Equipment:

Centeo TG40 System (by Centeo Biosciences) – designed to enable temperature optimisation for vapour phase diffusion protein crystallisation experiments. The Facility currently has 5 of those systems.

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A number of various stereo microscopes including two newly acquired SZX16 (by Olympus Ltd) are available for the Facility users. One of the key features of SZX16 includes the use of white LED illumination with average lifetime of 12,800 hours.

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Diffraction Data Storage:

ELARA, LEDA and EUROPA: Network-Attached Storage (NAS) devices for storage of collected X-ray diffraction images. Effective storage capacity per NAS: 12 Terabytes (8 x 2TB disks in RAID5).